Biology, Eighth Edition (Raven)
Learning OutcomesChapter 17
- Understand the importance of plasmids and viruses to genetic engineering.
- Know the natural function of restriction endonucleases and how a normal bacterial cell protects its DNA from their activity.
- Understand how “sticky ends” are formed and their importance to gene technology.
- Describe how a chimeric genome is constructed.
- Explain the four steps of genetic engineering experiments.
- Distinguish between the techniques of selection and screening of clones.
- Explain how to screen for clones that contain a desired gene fragment.
- Understand the value of and the processes involved with the polymerase chain reaction (PCR).
- Describe techniques used to characterize DNA.
- Discuss the different applications of gene technology.