Human Genetics: Concepts and Applications (Lewis), 9th Edition

Chapter 19: Genetic Technologies: Amplifying, Modifying, and Monitoring DNA

cDNA

View the animation below, then complete the quiz to test your knowledge of the concept.

1.		In order to clone eukaryotic DNA into prokaryotic cells
			A)	DNA with both exons and introns must be used
			B)	DNA without introns must be added
			C)	RNA with both exons and introns must be used
			D)	exons must be removed from eukaryotic DNA
			E)	introns must be added back to eukaryotic DNA
2.		In the process of cloning eukaryotic DNA into prokaryotic cells, the role of reverse transcriptase is to make
			A)	double-stranded eukaryotic cDNA from mature mRNA
			B)	mature mRNA from precursor mRNA
			C)	bacterial DNA from eukaryotic DNA
			D)	double-stranded DNA with introns added back
			E)	mRNA from DNA
3.		cDNA is
			A)	DNA with both introns and exons that can be cloned into prokaryotes
			B)	DNA with only introns that can be cloned into prokaryotes
			C)	eukaryotic DNA with only exons that can be cloned into prokaryotes
			D)	used to make precursor mRNA
4.		Prokaryotic DNA contains both exons and introns.
			A)	True
			B)	False
5.		RNA polymerase removes exons from precursor RNA.
			A)	True
			B)	False