1 A) recombinant DNA B) transgenic organisms C) biotechnology D) gene targeting 2 A) genomic DNA B) protein-encoding exons C) a gene variant D) All of the above 3 A) transversion B) transition C) transgenic D) transformant 4 A) a yeast used in industrial processes B) a bacterium able to metabolize components of crude oil C) a mouse that manufactures human protein in its milk D) Life forms cannot be patented. 5 A) Homo sapiens B) Drosophila melanogaster C) Thermus aquaticus D) Staphylococcus aureus 6 A) nucleic acid amplification B) polymerase chain reaction C) RNA amplification D) transcription-mediated amplification 7 A) always form "blunt" ends when cut by restriction enzymes B) are a type of symmetry in which nucleotides on complementary strands read the same in both directions C) are not useful in recombinant DNA experiments D) All of the above 8 A) codons for a mature mRNA B) sequences corresponding to promoters C) sequences corresponding to introns D) both b and c 9 A) recombinant DNA technology B) transgenic technology C) polymerase chain reaction D) gene targeting 10 A) restriction enzyme B) plasmid C) bacteriophage D) vector 11 A) DNA probes B) Homologous recombinants C) DNA libraries D) Knockout organisms 12 A) restriction enzyme B) plasmid C) bacteriophage D) chromosome 13 A) electroporation B) liposome transfer C) microinjection D) particle bombardment 14 A) protein B) amino acid C) DNA D) RNA 15 A) Restriction enzymes B) Cloning vectors C) DNA probes D) Gene targets 16 A) mRNA B) rRNA C) DNA D) plasmids 17 A) hemophilia B) dwarfism C) heart attack D) diabetes 18 A) recombinant DNA B) bioremediation C) rhizosecretion D) transgenic 19 A) electroporation B) liposome transfer C) microinjection D) particle bombardment 20 Ti plasmid is obtained from what species?A) Bacillus thuringiensis B) Escherichia coli C) Agrobacterium tumefaciens D) Thermus aquaticus 21 A) protection from viruses B) ligation of DNA sequences C) replication of DNA sequences D) transfer of DNA sequences from one cell to another 22 A) streptokinase B) tPA C) insulin D) penicillin 23 A) introduction of foreign DNA into cells B) gene expression profiling C) amplification of DNA sequences outside of cells 24 A) DNA variation screening B) gene expression profiling C) polymerase chain reaction D) antisense 25 A) cDNA molecules B) RNA molecules C) primers D) genomic libraries 26 A) True B) False 27 A) antibiotic resistance B) color changes in growth media C) All of the above 28 A) bioremediation B) transgenic C) polymerase chain reaction (PCR) D) microarray 29 A) knowing parts of a target DNA sequence to be amplified. B) two primers, complementary to each end of the target sequence. C) a large supply of DNA nucleotides. D) a heat-stable DNA polymerase. E) All of the above 30 A) heat is used to separate the two strands of the target DNA B) primers bind by complementary base pairing to the separated target strands C) TaqI DNA polymerase adds bases to the primers and builds a sequence complementary to the target sequence.
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