Human Genetics: Concepts and Applications (Lewis), 9th Edition

Chapter 19: Genetic Technologies: Amplifying, Modifying, and Monitoring DNA

Restriction Endonucleases

View the animation below, then complete the quiz to test your knowledge of the concept.

1.		Which of the following could NOT be the recognition site of a restriction endonculease?
			A)	GAATTC CTTAAG
			B)	ATCGAT TAGCTA
			C)	CTGCAG GACGTC
			D)	GCTTGC CGAACG
			E)	GGATCC CCTAGG
2.		The single-stranded ends of DNA molecules can be joined together by
			A)	restriction endonucleases.
			B)	DNA ligase.
			C)	DNA polymerase.
			D)	primase.
			E)	helicase.
3.		Human DNA cut with restriction enzyme A can be joined to
			A)	human DNA cut with restriction enzyme B.
			B)	human DNA that is uncut.
			C)	bacterial DNA cut with restriction enzyme A.
			D)	bacterial DNA that is uncut.
			E)	none of the above
4.		The joining of sticky ends involves the formation of phosphodiester bonds.
			A)	True
			B)	False
5.		Restriction enzymes cut only at specific sites and therefore are not useful for genetic engineering.
			A)	True
			B)	False