Human Genetics: Concepts and Applications (Lewis), 9th Edition

Chapter 19: Genetic Technologies: Amplifying, Modifying, and Monitoring DNA

Steps in Cloning a Gene

View the animation below, then complete the quiz to test your knowledge of the concept.

1.		Restriction enzymes do all of the following except...
			A)	fragment DNA.
			B)	produce staggered cuts in specific DNA sequences.
			C)	generate fragments with cohesive ends.
			D)	isolate and purify DNA.
			E)	cleave plasmids.
2.		Plasmids containing DNA fragment are incorporated into bacterial hosts by...
			A)	conjugation.
			B)	transduction.
			C)	transformation.
			D)	transposons.
			E)	vectors.
3.		Each of the different bacteria that contain the DNA fragments...
			A)	contain identical DNA fragments.
			B)	contain different DNA fragments.
			C)	contain the entire DNA library.
			D)	contain the desired cloned gene.
			E)	contain identical plasmids.
4.		The cells containing the are then plated on an agar medium.
			A)	True
			B)	False
5.		Since all of the resulting colonies are identical, the desired gene can then be isolated from each colony.
			A)	True
			B)	False